![SOLVED: Tris' is a buffer often used in biochemistry. We can simplify the equilibrium reaction as: Tris-HCl + H2O -> Cl- + H3O+ + Tris with pKa = 8.072 and the molar SOLVED: Tris' is a buffer often used in biochemistry. We can simplify the equilibrium reaction as: Tris-HCl + H2O -> Cl- + H3O+ + Tris with pKa = 8.072 and the molar](https://cdn.numerade.com/ask_images/5b13dce2a6f14d09b90f241d4290a50c.jpg)
SOLVED: Tris' is a buffer often used in biochemistry. We can simplify the equilibrium reaction as: Tris-HCl + H2O -> Cl- + H3O+ + Tris with pKa = 8.072 and the molar
STOCK SOLUTION RECIPIES: Tris-HCl Buffer 10X Tris-HCl (0.5M Tris Base, pH7.6): Trizma Base ---------------------------------- 6
![SOLVED: The composition of 1X TBE is: 89 mM Tris (molar mass = 121.14 g/mol), 89 mM Boric Acid (molar mass = 61.83 g/mol), 2 mM EDTA (molar mass = 292.24 g/mol). SOLVED: The composition of 1X TBE is: 89 mM Tris (molar mass = 121.14 g/mol), 89 mM Boric Acid (molar mass = 61.83 g/mol), 2 mM EDTA (molar mass = 292.24 g/mol).](https://cdn.numerade.com/ask_previews/45b69137-58da-4adb-8a28-4f556d28f8ec.gif)
SOLVED: The composition of 1X TBE is: 89 mM Tris (molar mass = 121.14 g/mol), 89 mM Boric Acid (molar mass = 61.83 g/mol), 2 mM EDTA (molar mass = 292.24 g/mol).
![SOLVED: 1. Describe how you would make the following two buffers (remember you need to calculate the mass of Tris base and the mass of Tris-acetate needed). Please show your work. a. SOLVED: 1. Describe how you would make the following two buffers (remember you need to calculate the mass of Tris base and the mass of Tris-acetate needed). Please show your work. a.](https://cdn.numerade.com/ask_previews/74769b2a-01c2-43be-a246-9b75219f8ccb_large.jpg)